中译英 3Q1.从前列腺癌PC-3细胞株中,以ALDH1标记、流式细胞仪分选出ALDH1A1阳性前列腺癌细胞,选取对数增

中译英 3Q
1.从前列腺癌PC-3细胞株中,以ALDH1标记、流式细胞仪分选出ALDH1A1阳性前列腺癌细胞,选取对数增长期的PC-3系ALDH1A1阳性细胞,予DMEM培养基将其培养成肿瘤微球体后平均分成四组,体外予不同剂量的盐霉素作用,将作用后的肿瘤微球体制成单细胞悬液,提取总RNA,运用microarray技术及实时荧光定量PCR技术检测PC-3系前列腺癌干细胞的全基因表达谱.
2.选取对数增长期的PC-3系ALDH1A1阳性细胞,无菌条件下接种到NOD/SCID小鼠双侧皮下,待移植瘤最大长径达1.5cm时,提取总RNA,运用microarray技术及实时荧光定量PCR技术检测盐霉素作用后PC-3系前列腺癌干细胞的全基因表达谱.

1.从前列腺癌PC-3细胞株中,以ALDH1标记、流式细胞仪分选出ALDH1A1阳性前列腺癌细胞,选取对数增长期的PC-3系ALDH1A1阳性细胞,予DMEM培养基将其培养成肿瘤微球体后平均分成四组,体外予不同剂量的盐霉素作用,将作用后的肿瘤微球体制成单细胞悬液,提取总RNA,运用microarray技术及实时荧光定量PCR技术检测PC-3系前列腺癌干细胞的全基因表达谱.
1.From prostate cancer three lines,PC - with ALDH1 mark,flow cytometric analysis ALDH1A1 sorting out positive prostate cancer cells,the selection of PC - 3 logarithmic growth of ALDH1A1 DMEM medium positive cells,to the after training to become tumor micro spheres in vitro average into four groups,to different doses of salt amphotericin role,will the tumor micro spheres role made single-celled levitation liquid extraction microarray technology,using total RNA and real-time fluorescence quantitative PCR technology testing PC - 3 department prostate cancer stem cells on the gene expression patterns of all.
2.选取对数增长期的PC-3系ALDH1A1阳性细胞,无菌条件下接种到NOD/SCID小鼠双侧皮下,待移植瘤最大长径达1.5cm时,提取总RNA,运用microarray技术及实时荧光定量PCR技术检测盐霉素作用后PC-3系前列腺癌干细胞的全基因表达谱.
2.Select the PC - 3 logarithmic growth of ALDH1A1 positive cells,aseptic conditions vaccination to NOD/SCID mice to transplant,bilateral subcutaneous tumor length-diameter of 150 largest cm,extraction microarray technology,using total RNA and real-time fluorescence quantitative PCR technology testing salt amphotericin role of PC - 3 after all the prostate cancer stem cells on the gene expression patterns.