英语翻译A useful screening method for the presence of PHAs is th
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英语翻译
A useful screening method for the presence of PHAs is the incorporation of the dye Nile Red into colonies on agar plates and its detection by fluorescence(Spiekermann et al.).The method has been used widely for therapid identification of PHA-producing environmental bacteria,but not yet with archaea.In our experiments,correct predictions from the presence or absence of fluorescent colonies for the production of PHAs were obtained also for haloarchaeal strains.The method can thus be considered compatible with agar plates containing media of high ionic strength.Other methods which were used here included staining for cytoplasmic granules of PHB in cells,which is fast and generally informative.
专业点
A useful screening method for the presence of PHAs is the incorporation of the dye Nile Red into colonies on agar plates and its detection by fluorescence(Spiekermann et al.).The method has been used widely for therapid identification of PHA-producing environmental bacteria,but not yet with archaea.In our experiments,correct predictions from the presence or absence of fluorescent colonies for the production of PHAs were obtained also for haloarchaeal strains.The method can thus be considered compatible with agar plates containing media of high ionic strength.Other methods which were used here included staining for cytoplasmic granules of PHB in cells,which is fast and generally informative.
专业点
一种有用的检测是否存在PHA的方法是 在琼脂板上将尼罗红(Nile Red)置入菌群并使用荧光检测(Spiekermann et al.).
这种方法被广泛地用于PHA快速鉴定PHA-producing environmental bacteria(产生PHA的环境细菌?我不是生物专业不清楚,你自己应该知道吧),但还没有应用于古生菌.
在我们的实验中,根据是否存在荧光的菌群 而获得了 关于PHA的生成物的正确预测,haloarchaeal strains(一种细菌,专业词汇)也是如此.
因此这种方法可以成为是与有高离子力介质琼脂板兼容的.
其他方法也被使用到了,包括在细胞中将PHB的细胞质颗粒染色,这种方法快速并且可以提供很多有用的信息.
这种方法被广泛地用于PHA快速鉴定PHA-producing environmental bacteria(产生PHA的环境细菌?我不是生物专业不清楚,你自己应该知道吧),但还没有应用于古生菌.
在我们的实验中,根据是否存在荧光的菌群 而获得了 关于PHA的生成物的正确预测,haloarchaeal strains(一种细菌,专业词汇)也是如此.
因此这种方法可以成为是与有高离子力介质琼脂板兼容的.
其他方法也被使用到了,包括在细胞中将PHB的细胞质颗粒染色,这种方法快速并且可以提供很多有用的信息.
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