搜搜考试网求细胞成像文献翻译 高手来
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求细胞成像文献翻译 高手来
In this work, we prepared [PtACHTUNGTRENUNG(C^N^N)Cl] complexes 1–3
(Figure 1) containing modified 6’-phenyl-2,2’-bipyridine ligands,
and found these complexes to have the following
properties: i) 3MLCT emission in the visible spectral region
(the long emission lifetime of phosphorescence can enhance
image signal stability and reduce the background noise arising
from fluorescence), ii) solubility and stability in aqueous
staining solutions at room temperature (thus the use of organic
solvents is not required for the optimal sensing of proteins),
and iii) binding to proteins via non-covalent interaction.
By employing these complexes, we have successfully
demonstrated the practical application of luminescent platinum(
II) complexes for protein staining and live cell imaging.
Common spectrophotometric methods, such as the Brad-
ACHTUNGTRENUNGford,[10a] bicinchoninic acid (BCA),[10b] and Lowry assays,[10c]
which are used for protein quantification, in general are
hampered by the interfering agents found in the sample
preparation buffers, require relatively large sample volumes,
have limited dynamic range, and exhibit responses which
are affected by the structure of proteins, the amino acid sequence,
and the isoelectric point (pI). Thus the developmentof analytical tools to detect proteins with high sensitivity,
good binding linearity, small protein-to-protein variation,
and low consumption of samples is of particular importance
in biochemistry.
In this work, we prepared [PtACHTUNGTRENUNG(C^N^N)Cl] complexes 1–3
(Figure 1) containing modified 6’-phenyl-2,2’-bipyridine ligands,
and found these complexes to have the following
properties: i) 3MLCT emission in the visible spectral region
(the long emission lifetime of phosphorescence can enhance
image signal stability and reduce the background noise arising
from fluorescence), ii) solubility and stability in aqueous
staining solutions at room temperature (thus the use of organic
solvents is not required for the optimal sensing of proteins),
and iii) binding to proteins via non-covalent interaction.
By employing these complexes, we have successfully
demonstrated the practical application of luminescent platinum(
II) complexes for protein staining and live cell imaging.
Common spectrophotometric methods, such as the Brad-
ACHTUNGTRENUNGford,[10a] bicinchoninic acid (BCA),[10b] and Lowry assays,[10c]
which are used for protein quantification, in general are
hampered by the interfering agents found in the sample
preparation buffers, require relatively large sample volumes,
have limited dynamic range, and exhibit responses which
are affected by the structure of proteins, the amino acid sequence,
and the isoelectric point (pI). Thus the developmentof analytical tools to detect proteins with high sensitivity,
good binding linearity, small protein-to-protein variation,
and low consumption of samples is of particular importance
in biochemistry.
在本文中,我们制备了含有改性6’苯基-2,2’二吡啶配体的 络合物1－3（图1）,并且发现,这些络合物具有以下性质：1）在可见光光谱范围的3MLCT发射（磷光现象的长发射寿命会提高图像信号的稳定性并降低因荧光产生的背景噪声）；2）在室温下于含水染色溶液中的可溶性和稳定性（因此不需要使用有机溶剂实现蛋白质的最佳传感）；3）通过非共价互作用与蛋白质结合.通过采用这些络合物,我们已经成功地演示了发光铂（II）络合物在蛋白质染色和活细胞成像中的应用.用于蛋白质的定量的常用分光光度法,如Bradford、[10a] 双喹啉-4-羧酸(BCA),[10b]和Lowry 的试验,[10c] 一般来说会受到在样本制备缓冲剂中发现的干扰剂的阻碍,所以要求相对较大的样本量,有有限的动态范围,而且显示出受到蛋白质结构、氨基酸序列、以及等电点（pI）影响的响应.因此,开发具有高灵敏度、良好结合线性度、小的蛋白质－蛋白质变化、和低样本消耗的检测蛋白质的分析工具在生物化学领域具有特别的重要性.